Sherlock Holmes investigates a series of so-called "pajama suicides". He knows the female villain behind them is as cunning as Moriarty and as venomous as a spider.
- Basil Rathbone as Sherlock Holmes
- Nigel Bruce as Dr. John Watson
- Gale Sondergaard as Adrea Spedding
- Vernon Downing as Norman Locke
- Dennis Hoey as Inspector Lestrade
- Alec Craig as Radlik
- Arthur Hohl as Adam Gilflower
- Mary Gordon as Mrs Hudson
- Teddy Infuhr as Larry
- Angelo Rossitto as The Pygmy
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